replication origin การใช้

• They are consisted mostly of LADs and contain late replication origins.
• By firing of replication origins, controlled spatially and temporally, the formation of replication foci is regulated.
• As well, they are typically made up of self-interacting domains and contain early replication origins.
• That is, couples of replication factories are loaded on replication origins and the factories associated with each other.
• DNA replication is initiated from specific sequences called origins of replication, and eukaryotic cells have multiple replication origins.
• Ctf4 is a polymerase ? accessory factor, which is required for the recruitment of polymerase ? to replication origins.
• In eukaryotic cells, termination of replication usually occurs through the collision of the two replicative forks between two active replication origins.
• GC or AT skew changes sign at the boundaries of the two replichores, which correspond to DNA replication origin or terminus.
• Additionally, Dbf4 stimulates Cell division cycle 7-related protein kinase ( Cdc7 ) activity, which promotes activation of replication origins.
• It catalyzes the unwinding of double-stranded plasmid DNA that has been nicked at the replication origin by the replication initiation protein.
• The complex formed at the replication origin also includes several DNA-binding proteins-Hu, IHF and FIS that facilitate DNA bending.
• Leading strand synthesis begins with the synthesis of a short RNA primer at the replication origin by the enzyme Primase ( DnaG protein ).
• Active S cyclin-CDK complexes phosphorylate proteins that make up the pre-replication complexes assembled during G 1 phase on DNA replication origins.
• Failure to maintain control over pre-RC formation at replication origins results in a phenomenon known as  rereplication which is common in cancer cells.
• Clb5, 6-Cdk1 complexes directly lead to replication origin initiation; however, they are inhibited by Sic1, preventing premature S phase initiation.
• A "'minichromosome "'is a small chromatin-like structure consisting of centromeres, telomeres and replication origins and little additional genetic material.
• They detected DNA replication of pairs of the tagged loci spaced apart symmetrically from a replication origin and found that the distance between the pairs decreased markedly by time.
• The Heun s results denied the traditional concepts, budding yeasts don't have lamins, and support that replication origins self-assemble and form replication foci.
• It binds directly to replication origin recognition complexes ( ORCs ) that are primarily associated with the terminal repeat ( TR ) region of the HHV-8 genome.
• As other mechanism of the rescue there is application of "'dormant replication origins "'that excess origins don't fire in normal DNA replication.